How to make Canine Semen Extender
1. Tris(hydroxymethyl)aminomethane 3.025 g
2. Citric acid 1.7 g
3. Fructose 1.25 g
4. Distilled water to 100 ml
5. Egg yolk 20 ml
6. Benzylpenicillin 1 mg/ml
7. Dihydrostreptomycin sulphate 1 mg/ml
If it is prepared without egg yolk, the extender can be stored in smaller portions in an ordinary freezer, and 20% egg yolk can be added at the time of use. If it is freeze-stored containing
egg yolk it shoud probably be used within 2 to 3 months.
This formula works really well I have stored semen for up to 20 days in the fridge changing the extender every 5 days, and the semen starts right back up again all you have to do is put the semen in a water bath at 98.6 Fahrenheit = 37 Celsius
If you need to store semen just for a couple of days, all you need to do is put it in either egg yolk of skim milk and both work good for a few days, and if you need to get more technical than use my formula above.
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